4M7C
Crystal structure of the TRF2-binding motif of SLX4 in complex with the TRFH domain of TRF2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-03 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.591, 69.183, 118.078 |
| Unit cell angles | 90.00, 94.73, 90.00 |
Refinement procedure
| Resolution | 44.860 - 2.050 |
| R-factor | 0.2165 |
| Rwork | 0.214 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3bu8 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.866 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 100.000 | 100.000 | 2.120 |
| High resolution limit [Å] | 2.050 | 4.420 | 2.050 |
| Rmerge | 0.068 | 0.036 | 0.287 |
| Number of reflections | 32186 | ||
| <I/σ(I)> | 9.2 | ||
| Completeness [%] | 96.7 | 98 | 95.6 |
| Redundancy | 3.9 | 3.9 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 277 | 10% PEG 4000, 0.1M HEPES, pH 7.4, 100mM NaCl, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
