4M62
Ontogeny of recognition specificity and functionality for the anti-HIV neutralizing antibody 4E10
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-16 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.880, 103.480, 109.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 68.880 - 1.800 |
| R-factor | 0.19076 |
| Rwork | 0.189 |
| R-free | 0.21608 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.699 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 68.880 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 71260 | |
| Completeness [%] | 98.0 | 98 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | 0.1M Ac, pH 5.0, 1.6M Li2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
