4LTC
Crystal structure of yeast 20S proteasome in complex with enone carmaphycin analogue 6
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-07-05 |
| Detector | PSI PILATUS 6M |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 135.170, 300.230, 144.380 |
| Unit cell angles | 90.00, 112.79, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.500 |
| R-factor | 0.2055 |
| Rwork | 0.204 |
| R-free | 0.23250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.910 |
| Data scaling software | XSCALE |
| Phasing software | CNS |
| Refinement software | REFMAC (5.7.0032) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 2.600 | ||
| High resolution limit [Å] | 2.500 | 30.000 | 2.500 |
| Rmerge | 0.051 | 0.017 | 0.538 |
| Number of reflections | 354256 | 112 | 39325 |
| <I/σ(I)> | 16.34 | 79.32 | 1.71 |
| Completeness [%] | 97.1 | 49.1 | 97.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 297 | 30 mM of magnesium acetate, 100 mM of MES (pH 7.2) and 12% of MPD, vapor diffusion, temperature 297K |
