4LRL
Structure of an Enterococcus Faecalis HD-domain protein complexed with dGTP and dTTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97872 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 91.541, 144.625, 155.560 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.860 - 2.350 |
| R-factor | 0.18693 |
| Rwork | 0.184 |
| R-free | 0.23461 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3irh |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.153 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.074 | 0.430 |
| Number of reflections | 86389 | |
| <I/σ(I)> | 25.9 | 2.1 |
| Completeness [%] | 99.4 | 94.9 |
| Redundancy | 4.2 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 295 | 0.1 M MIB buffer (pH 5) and 25 % w/v PEG 1500, VAPOR DIFFUSION, SITTING DROP, temperature 295K |
