4LK3

Crystal structure of Human UDP-xylose synthase R236A substitution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2009-06-05
Detector	MARMOSAIC 225 mm CCD
Spacegroup name	P 21 21 21
Unit cell lengths	83.570, 92.070, 290.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.370 - 2.640
R-factor	0.2
Rwork	0.198
R-free	0.24500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b69
RMSD bond length	0.011
RMSD bond angle	1.481
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.710
High resolution limit [Å]	2.640	2.640
Rmerge	0.092	0.866
Number of reflections	65871
<I/σ(I)>	19.9	2.7
Completeness [%]	98.4	86.2
Redundancy	11.87

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.4	293	0.1 M ammonium sulfate, 0.1 M succinate, 10% DMSO, pH 5.4, Vapor diffusion, hanging drop, temperature 293K