4LIQ

Structure of the extracellular domain of human CSF-1 receptor in complex with the Fab fragment of RG7155

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	110
Detector technology	PIXEL
Collection date	2010-08-20
Detector	PSI PILATUS 6M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	82.171, 113.580, 146.625
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.070 - 2.600
R-factor	0.189
Rwork	0.186
R-free	0.23680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ejj 2ec8
RMSD bond length	0.009
RMSD bond angle	1.190
Data reduction software	XDS
Data scaling software	SADABS
Phasing software	PHASER
Refinement software	BUSTER (2.11.4)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	145.000	2.650
High resolution limit [Å]	2.550	2.550
Rmerge	0.119	0.780
Number of reflections	45526
<I/σ(I)>		1.4
Completeness [%]	99.9	100
Redundancy	6.7	6.93

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	25% PEG 3350, 0.2M lithium sulfate, 0.1M HEPES, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K