4LF3

Inhibitory Mechanism of an Allosteric Antibody Targeting the Glucagon Receptor

Experimental procedure

Experimental method	SINGLE
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-25
Detector	ADSC
Wavelength(s)	0.97946
Spacegroup name	P 1 21 1
Unit cell lengths	104.928, 62.777, 116.432
Unit cell angles	90.00, 106.04, 90.00

Refinement procedure

Resolution	55.950 - 2.735
R-factor	0.2116
Rwork	0.209
R-free	0.26350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4ers
RMSD bond length	0.010
RMSD bond angle	1.538
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.16)
Phasing software	PHASER (2.1.4)
Refinement software	PHENIX (1.7.1_743)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	55.950	55.950	2.880
High resolution limit [Å]	2.735	8.650	2.735
Rmerge		0.022	0.367
Total number of observations		3726	13662
Number of reflections	35933
<I/σ(I)>	12.6	27.1	2.1
Completeness [%]	92.6	88.4	92.4
Redundancy	2.9	3.2	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	289	0.1 M Tris-HCl, 0.2 M NaCl, PEG3350, pH 8.5, vapor diffusion, hanging drop, temperature 289K