4LEZ

Structure of mouse cGAS bound to an 18bp DNA and cGAS product

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	153
Detector technology	IMAGE PLATE
Collection date	2013-05-01
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.542
Spacegroup name	P 21 21 21
Unit cell lengths	79.209, 99.031, 142.631
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.549 - 2.360
R-factor	0.1946
Rwork	0.192
R-free	0.24660
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.229
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.2_1309))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.500	2.430
High resolution limit [Å]	2.360	2.360
Rmerge	0.069
Number of reflections	45447
Completeness [%]	96.6	97
Redundancy	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	277	24-36% MPD, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K