4L8W
Crystal structure of gamma glutamyl hydrolase (H218N) from zebrafish complex with MTX polyglutamate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2012-06-10 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 225.591, 60.409, 157.055 |
| Unit cell angles | 90.00, 100.98, 90.00 |
Refinement procedure
| Resolution | 29.630 - 2.390 |
| R-factor | 0.19621 |
| Rwork | 0.193 |
| R-free | 0.25756 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1l9x |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.833 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | X-PLOR |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.470 |
| High resolution limit [Å] | 2.390 | 2.390 |
| Number of reflections | 82119 | |
| Completeness [%] | 99.0 | 99 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 20% PEG 3350, 8% Tassimate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
