4KXZ
crystal structure of tgfb2 in complex with GC2008.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-01-25 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.98011 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 131.200, 359.680, 64.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.730 - 2.830 |
| Rwork | 0.183 |
| R-free | 0.22200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.190 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.730 | 48.730 | 3.000 |
| High resolution limit [Å] | 2.830 | 8.370 | 2.830 |
| Rmerge | 0.121 | 0.052 | 0.703 |
| Number of reflections | 74205 | ||
| <I/σ(I)> | 32.17 | 3 | |
| Completeness [%] | 99.5 | 99 | 97.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | 296 | 35% PEG400, 100mM MES, VAPOR DIFFUSION, temperature 296K, pH 6.0 |
