4KU8
Structures of PKGI Reveal a cGMP-Selective Activation Mechanism
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-12-20 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 0.97931 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 78.766, 78.766, 147.001 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.107 - 1.994 |
R-factor | 0.1997 |
Rwork | 0.197 |
R-free | 0.24470 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.006 |
RMSD bond angle | 0.980 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.7.2_869)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 36.900 | 2.048 |
High resolution limit [Å] | 1.994 | 1.994 |
Number of reflections | 36516 | |
Completeness [%] | 99.4 | 96.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.5 | 277 | 0.1M SPG buffer, 25% PEG1500, pH 5.5, VAPOR DIFFUSION, temperature 277K |