4KRM

Nanobody/VHH domain 7D12 in complex with domain III of the extracellular region of EGFR, pH 3.5

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	CCD
Collection date	2009-07-10
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.980
Spacegroup name	P 21 21 21
Unit cell lengths	78.702, 147.245, 254.799
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.514 - 2.655
R-factor	0.2099
Rwork	0.208
R-free	0.24430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ENTRIES 3B2U AND 4KRN
RMSD bond length	0.005
RMSD bond angle	0.855
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX (1.8.2_1309)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.700
High resolution limit [Å]	2.650	7.190	2.650
Rmerge	0.108	0.044	0.614
Number of reflections	83116
<I/σ(I)>	8.5
Completeness [%]	96.6	99.8	75.4
Redundancy	6.8	7	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	3.5	293	22.5% PEG3350, 0.1 M sodium citrate, pH 3.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K