4KGQ
Crystal structure of a human light loop mutant in complex with dcr3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-04-18 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.075 |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 149.269, 149.269, 149.269 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.350 - 2.270 |
| R-factor | 0.19705 |
| Rwork | 0.195 |
| R-free | 0.23803 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4j6g |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.099 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.310 |
| High resolution limit [Å] | 2.270 | 2.270 |
| Rmerge | 0.104 | 0.650 |
| Number of reflections | 51516 | |
| <I/σ(I)> | 23.091 | 4.429 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.6 | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 290 | 0.1 M Imidazole:HCl, pH 8.0, 2.5 M Sodium Chloride, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 290K |
