4KFG
The DNA Gyrase B ATP binding domain of Escherichia coli in complex with a small molecule inhibitor.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-10 |
| Detector | NOIR-1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.894, 82.477, 53.740 |
| Unit cell angles | 90.00, 100.16, 90.00 |
Refinement procedure
| Resolution | 44.530 - 1.600 |
| R-factor | 0.19381 |
| Rwork | 0.192 |
| R-free | 0.22096 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kzn |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.559 |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.530 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 54029 | |
| Completeness [%] | 99.7 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 15% PEG 3350 Tris pH 7.5, 0.2M AmSO4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
