4K94
Crystal structure of KIT D4D5 fragment in complex with anti-Kit antibody Fab19
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-06-27 |
| Detector | PSI PILATUS 6M |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 169.580, 48.980, 106.970 |
| Unit cell angles | 90.00, 122.38, 90.00 |
Refinement procedure
| Resolution | 45.495 - 2.400 |
| R-factor | 0.1998 |
| Rwork | 0.198 |
| R-free | 0.23490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.770 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 24912 | |
| <I/σ(I)> | 11 | 1.2 |
| Completeness [%] | 84.5 | 64.1 |
| Redundancy | 3.1 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 294 | 13% PEG 3350, 0.5 M magnesium chloride, 0.1 M Tris-HCl, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
