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4K8V

Structure of cyclic GMP-AMP Synthase (cGAS)

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsNSLS BEAMLINE X29A
Synchrotron siteNSLS
BeamlineX29A
Temperature [K]200
Detector technologyCCD
Collection date2013-01-23
DetectorADSC QUANTUM 315r
Wavelength(s)0.9790
Spacegroup nameP 1 21 1
Unit cell lengths86.563, 84.147, 124.716
Unit cell angles90.00, 92.74, 90.00
Refinement procedure
Resolution44.070 - 2.000
R-factor0.177
Rwork0.175
R-free0.20800
Structure solution methodSAD
RMSD bond length0.010
RMSD bond angle1.207
Data reduction softwareHKL-2000
Data scaling softwareHKL-2000
Phasing softwareSOLVE
Refinement softwarePHENIX ((phenix.refine: 1.8.2_1309))
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.070
High resolution limit [Å]2.0002.000
Number of reflections118611
Completeness [%]98.198.6
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, HANGING DROP7.62770.1 M HEPES, 0.1 M MgAc2, 20% PEG3350, pH 7.6, VAPOR DIFFUSION, HANGING DROP, temperature 277K

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