4K6I
Crystal structure of human retinoid X receptor alpha-ligand binding domain complex with Targretin and the coactivator peptide GRIP-1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU R-AXIS IV |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-09-01 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.315 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 68.150, 68.150, 106.590 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.300 - 2.100 |
R-factor | 0.246 |
Rwork | 0.246 |
R-free | 0.28800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3oap |
RMSD bond length | 0.009 |
RMSD bond angle | 1.162 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.300 | 2.180 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.078 | |
<I/σ(I)> | 14.1 | 4.5 |
Completeness [%] | 96.9 | 99.7 |
Redundancy | 8.12 | 7.73 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 5-15% PEG4000, 6-12% Glycerol, 0.1M Bis-Tris pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |