4K0V

Structural basis for angiopoietin-1 mediated signaling initiation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-10
Detector	ADSC QUANTUM 315
Spacegroup name	I 41 2 2
Unit cell lengths	189.533, 189.533, 334.867
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.012 - 4.510
R-factor	0.2496
Rwork	0.248
R-free	0.28780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gy7
RMSD bond length	0.011
RMSD bond angle	1.625
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall
Low resolution limit [Å]	30.012
High resolution limit [Å]	4.510
Number of reflections	18062
<I/σ(I)>	17.5
Completeness [%]	98.5
Redundancy	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	293	1.6 M NaH2PO4, 0.4 M K2HPO4, 0.1 M phosphate-citrate, pH 4.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K