4JVF
The Crystal structure of PDE6D in complex with the inhibitor (s)-5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2012-03-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 55.910, 55.910, 116.670 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 27.900 - 2.400 |
R-factor | 0.20949 |
Rwork | 0.207 |
R-free | 0.25659 |
Starting model (for MR) | 3t5g |
RMSD bond length | 0.022 |
RMSD bond angle | 1.980 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MOLREP |
Refinement software | REFMAC (5.6.0093) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.900 | 2.500 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.122 | |
Number of reflections | 8659 | |
Completeness [%] | 99.0 | 98.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 293 | 18% PEG 4000, 25mM Sodium Acetate, 200mM Ammonium Sulphate, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K |