4JKP

Restricting HIV-1 Pathways for Escape using Rationally-Designed Anti-HIV-1 Antibodies

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-03-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	69.330, 70.490, 232.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.846 - 2.820
R-factor	0.1953
Rwork	0.193
R-free	0.23100
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.027
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: dev_1026))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.850	2.890
High resolution limit [Å]	2.820	2.820
Number of reflections	27846
Completeness [%]	98.4	99.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	12% (v/v) isopropanol, 10% (w/v) polyethylene glycol 10,000 kD, 0.1 M citrate , pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K