4IW3

Crystal structure of a Pseudomonas putida prolyl-4-hydroxylase (P4H) in complex with elongation factor Tu (EF-Tu)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	CCD
Collection date	2012-09-23
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979500
Spacegroup name	P 31 2 1
Unit cell lengths	200.704, 200.704, 74.833
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.558 - 2.697
R-factor	0.1705
Rwork	0.168
R-free	0.21810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1dg1 4j25
RMSD bond length	0.011
RMSD bond angle	1.215
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.1_1168)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	29.558	29.558	2.860
High resolution limit [Å]	2.697	7.830	2.700
Rmerge	0.086	0.013	0.013
Rmeas		0.031	1.437
Number of reflections	47788	3729	14842
<I/σ(I)>	13.37	39.98	1.7
Completeness [%]	99.8	98	99.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	0.8mM MnCl2, 0.9mM OGA, 1mM GDP, 0.1M MgCl2, 22% polyacrylate 5100, 0.1M Hepes pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K