4IFD
Crystal structure of an 11-subunit eukaryotic exosome complex bound to RNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-10-28 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.9199 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 154.090, 107.440, 150.460 |
Unit cell angles | 90.00, 110.63, 90.00 |
Refinement procedure
Resolution | 49.638 - 2.805 |
R-factor | 0.1846 |
Rwork | 0.182 |
R-free | 0.22390 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2nn6 2wp8 2ja9 2je6 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.542 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.640 | 49.640 | 3.000 |
High resolution limit [Å] | 2.805 | 20.000 | 2.800 |
Rmerge | 0.176 | 0.069 | 0.548 |
Number of reflections | 99137 | ||
<I/σ(I)> | 12.25 | 35.76 | 1.89 |
Completeness [%] | 87.4 | 91.2 | 56.5 |
Redundancy | 12.8 | 15.76 | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 292 | 0.02M Tris pH7.5, 0.05M NaCl, 0.001M MnCl2, 0.002M MgCl2, 1mM TCEP, 0.15M MES pH6.5, 0.27M NaBr, 11.4-12.2% PEG 3350, VAPOR DIFFUSION, SITTING DROP, temperature 292K |