4IF8
Structure Of Vaspin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9184 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 133.550, 152.100, 61.450 |
| Unit cell angles | 90.00, 97.52, 90.00 |
Refinement procedure
| Resolution | 29.800 - 2.080 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qlp |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.080 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.8.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 29.800 |
| High resolution limit [Å] | 2.080 |
| Rmerge | 0.051 |
| Number of reflections | 72713 |
| Completeness [%] | 99.9 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.1 | 292 | 0.1M NA CITRATE, 9% PEG4000, 0.1M Ammonia sulfate, pH 5.1, Vapor Diffusion, Hanging Drop, temperature 292K |
