4I0K

Crystal structure of murine B7-H3 extracellular domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X29A
Synchrotron site	NSLS
Beamline	X29A
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-19
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9789
Spacegroup name	P 61 2 2
Unit cell lengths	100.920, 100.920, 188.180
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.000 - 2.970
R-factor	0.21712
Rwork	0.215
R-free	0.24974
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3bis
RMSD bond length	0.012
RMSD bond angle	1.742
Data reduction software	MOSFLM
Data scaling software	SCALA (3.3.20)
Phasing software	BALBES
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	87.399	48.738	3.130
High resolution limit [Å]	2.833	9.390	2.970
Rmerge		0.041	0.800
Number of reflections	12250
<I/σ(I)>	18.1	14.9	1
Completeness [%]	99.7	97.5	100
Redundancy	13.1	11.4	13.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	291	0.1 M HEPES, pH 7.5; 20% PEG-10000, VAPOR DIFFUSION, temperature 291K
1	VAPOR DIFFUSION	7.5	291	0.1 M HEPES, pH 7.5; 20% PEG-10000, VAPOR DIFFUSION, temperature 291K