4HEL
Crystal structure analysis of apo-GroEL structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-09 |
| Detector | MARCCD225 |
| Wavelength(s) | 0.97327 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 136.240, 261.830, 282.280 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.380 - 3.200 |
| R-factor | 0.19927 |
| Rwork | 0.198 |
| R-free | 0.22333 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kp8 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.416 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 59.400 | 3.370 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.320 | 0.850 |
| Number of reflections | 166588 | |
| <I/σ(I)> | 6.1 | 2.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.5 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 32% MPD, 100mM Tris-HCl, 160mM MgCl2, 10% Glycerol, 2%(w/v) PEG6000, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
