4HDA
Crystal structure of human Sirt5 in complex with Fluor-de-Lys peptide and resveratrol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | OTHER |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-03-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.300, 112.690, 55.890 |
| Unit cell angles | 90.00, 90.05, 90.00 |
Refinement procedure
| Resolution | 38.778 - 2.601 |
| R-factor | 0.2046 |
| Rwork | 0.199 |
| R-free | 0.25210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.325 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.800 | 2.760 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.075 | 0.450 |
| Number of reflections | 15354 | |
| <I/σ(I)> | 14.5 | 3 |
| Completeness [%] | 97.6 | 93.4 |
| Redundancy | 3.6 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 150 mM NH4Cl, 15% (w/v) PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
