4HBN
Crystal structure of the human HCN4 channel C-terminus carrying the S672R mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-12-21 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54 |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 95.467, 95.467, 115.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.600 |
| R-factor | 0.25941 |
| Rwork | 0.257 |
| R-free | 0.29669 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.238 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | PHASES |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 29.170 | 2.690 |
| High resolution limit [Å] | 2.600 | 5.590 | 2.600 |
| Rmerge | 0.019 | 0.232 | |
| Number of reflections | 8484 | ||
| <I/σ(I)> | 65.4 | 7.6 | |
| Completeness [%] | 99.1 | 93.7 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 277 | 1.6 M KH2PO4/Na2HPO4 and 0.1 M Phosphate-Citrate, pH 4.2, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
