4H7U
Crystal structure of pyranose dehydrogenase from Agaricus meleagris, wildtype
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-5 |
| Synchrotron site | MAX II |
| Beamline | I911-5 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-17 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.90772 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.938, 74.640, 139.286 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.700 - 1.600 |
| R-factor | 0.17176 |
| Rwork | 0.171 |
| R-free | 0.20116 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jbv |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.853 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | BALBES |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.700 | 1.700 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 72035 | |
| <I/σ(I)> | 16.4 | 2.6 |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 7.3 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 50 mM KH2PO4, 5% 2-methyl-2,4-pentanediol, 20% (w/v) polyethylene glycol 8000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
