4H54
Crystal structure of the diguanylate cyclase DgcZ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-08-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 61 |
| Unit cell lengths | 102.593, 102.593, 129.156 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 73.200 - 3.900 |
| R-factor | 0.2421 |
| Rwork | 0.242 |
| R-free | 0.24110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 3T90 AND 3TVK |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.040 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 89.000 | 89.050 | 4.110 |
| High resolution limit [Å] | 3.900 | 12.330 | 3.900 |
| Rmerge | 0.030 | 0.770 | |
| Number of reflections | 7076 | ||
| <I/σ(I)> | 15.45 | 1 | |
| Completeness [%] | 99.9 | 99.06 | 99.87 |
| Redundancy | 13.38 | 7.93 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 200 mM calcium acetate, 20.0% PEG4000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
