4GYY

Crystal structure of human O-GlcNAc Transferase with UDP-5SGlcNAc and a peptide substrate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-12-11
Detector	PSI PILATUS 6M
Wavelength(s)	1.000
Spacegroup name	I 1 2 1
Unit cell lengths	98.520, 136.480, 152.770
Unit cell angles	90.00, 103.51, 90.00

Refinement procedure

Resolution	59.946 - 1.850
R-factor	0.2187
Rwork	0.218
R-free	0.23740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.806
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHENIX (1.7.2_869)
Refinement software	PHENIX ((phenix.refine: 1.7.2_869))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.950	1.950
High resolution limit [Å]	1.850	1.850
Number of reflections	166242
Completeness [%]	99.7	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	1.6M Lithium Sulfate, 0.1M Bis Tris Propane pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K