4GTY
Crystal structure of mouse Enpp1 in complex with GMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-06-22 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 31 |
| Unit cell lengths | 104.863, 104.863, 174.831 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.047 - 3.190 |
| R-factor | 0.2593 |
| Rwork | 0.258 |
| R-free | 0.28080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4gtw |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.854 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 3.260 |
| High resolution limit [Å] | 3.190 | 8.670 | 3.200 |
| Rmerge | 0.169 | 0.046 | 0.521 |
| Number of reflections | 35428 | ||
| <I/σ(I)> | 6.9 | ||
| Completeness [%] | 99.0 | 99.6 | 98.4 |
| Redundancy | 6.5 | 10.8 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.5 | 293 | PEG 600, MgOAc, NaCl, ZnSO4, pH 4.5, vapor diffusion, temperature 293K |
