4GIW
Crystal structure of the RUN domain of human NESCA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-06-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97915 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 39.116, 94.201, 44.602 |
Unit cell angles | 90.00, 99.54, 90.00 |
Refinement procedure
Resolution | 35.700 - 2.000 |
R-factor | 0.20488 |
Rwork | 0.204 |
R-free | 0.21233 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.098 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.700 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.052 | 0.103 |
Number of reflections | 20352 | |
<I/σ(I)> | 2.8 | |
Completeness [%] | 99.6 | 99.42 |
Redundancy | 7.247 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 20%(w/v) PEG3350, 0.2M K2HPO4, VAPOR DIFFUSION, SITTING DROP, temperature 289K |