4G8L
Active state of intact sensor domain of human RNase L with 2-5A bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Collection date | 2012-03-11 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.072 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.980, 164.110, 86.220 |
| Unit cell angles | 90.00, 100.20, 90.00 |
Refinement procedure
| Resolution | 38.750 - 2.800 |
| R-factor | 0.2299 |
| Rwork | 0.228 |
| R-free | 0.26090 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4g8k |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.985 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 39.000 | 40.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 30.000 | 2.800 |
| Number of reflections | 42322 | ||
| Completeness [%] | 99.8 | 95 | 99.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7 | 293 | 200 mM sodium acetate, 18% PEG 3350, 1.3M sodium citrate, pH 7.0, VAPOR DIFFUSION, temperature 293K |
