4G2C
DyP2 from Amycolatopsis sp. ATCC 39116
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-03-06 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.116 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 48.131, 77.952, 252.353 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.040 - 2.250 |
R-factor | 0.1867 |
Rwork | 0.184 |
R-free | 0.24320 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2d3q |
RMSD bond length | 0.013 |
RMSD bond angle | 1.104 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.7.3_928)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.040 | 2.370 |
High resolution limit [Å] | 2.250 | 2.250 |
Rmerge | 0.088 | 0.556 |
Number of reflections | 45753 | |
<I/σ(I)> | 13 | 2.4 |
Completeness [%] | 98.9 | 95 |
Redundancy | 4.4 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 291 | 100 mM sodium acetate, pH 5.0, 22 mM manganese chloride, 8% PEG4000, VAPOR DIFFUSION, HANGING DROP, temperature 291K |