4FM9

Human topoisomerase II alpha bound to DNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-04-28
Detector	ADSC QUANTUM 210
Wavelength(s)	1.1159
Spacegroup name	C 2 2 21
Unit cell lengths	85.918, 215.081, 128.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.611 - 2.901
R-factor	0.2291
Rwork	0.227
R-free	0.27020
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.847
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER (2.1.4)
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	80.000	80.000	3.000
High resolution limit [Å]	2.900	6.250	2.900
Rmerge	0.093	0.044	0.712
Number of reflections	25479
<I/σ(I)>	11.8
Completeness [%]	95.3	93.3	91.6
Redundancy	7.2	8.2	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	10% PEG 3000, 100mM Na-cacodylate, 200mM MgCl2, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K