4FLO
Crystal structure of Amylosucrase double mutant A289P-F290C from Neisseria polysaccharea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-13 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.93340 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 96.030, 115.980, 60.460 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 13.990 - 2.200 |
| R-factor | 0.1691 |
| Rwork | 0.164 |
| R-free | 0.21580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g5a |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.067 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.660 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.101 | 0.306 |
| Number of reflections | 35028 | |
| <I/σ(I)> | 7.2 | 2.5 |
| Completeness [%] | 99.8 | 99.1 |
| Redundancy | 4.7 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 285 | 30% PEG 6000, 0.1 M HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 285.0K |
