4FK5
Structure of the SAGA Ubp8(S144N)/Sgf11/Sus1/Sgf73 DUB module
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 108 |
| Detector technology | CCD |
| Collection date | 2010-08-14 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 83.528, 104.775, 107.018 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 74.868 - 2.032 |
| R-factor | 0.17669 |
| Rwork | 0.175 |
| R-free | 0.21203 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.277 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.868 | 2.070 |
| High resolution limit [Å] | 2.032 | 2.032 |
| Rmerge | 0.865 | |
| Number of reflections | 60890 | |
| <I/σ(I)> | 4.64 | |
| Completeness [%] | 99.5 | 100 |
| Redundancy | 14.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.2 | 291 | sodium citrate pH 5.2, 16% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
