4FIP

Structure of the SAGA Ubp8(S144N)/Sgf11(1-72, Delta-ZnF)/Sus1/Sgf73 DUB module

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	108
Detector technology	CCD
Collection date	2010-11-08
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0332
Spacegroup name	P 21 21 21
Unit cell lengths	75.973, 79.975, 274.259
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.180 - 2.686
R-factor	0.18717
Rwork	0.183
R-free	0.26361
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3mhs
RMSD bond length	0.012
RMSD bond angle	1.545
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.740
High resolution limit [Å]	2.686	2.686
Rmerge	0.151	0.637
Number of reflections	43495
<I/σ(I)>	12.227	2.345
Completeness [%]	91.9	84.9
Redundancy	7.8	6.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	291	14% PEG3350, 0.18-0.22 M Tri-ammonium citrate, 8-14% glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 291K