4FIC
Kinase domain of cSrc in complex with a hinge region-binding fragment
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 90 |
| Detector technology | PIXEL |
| Collection date | 2012-02-13 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.979400 |
| Spacegroup name | P 1 |
| Unit cell lengths | 42.034, 63.013, 73.561 |
| Unit cell angles | 79.63, 88.12, 90.17 |
Refinement procedure
| Resolution | 43.360 - 2.500 |
| R-factor | 0.1997 |
| Rwork | 0.198 |
| R-free | 0.24390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oiq |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.473 |
| Data scaling software | XSCALE |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.600 | |
| High resolution limit [Å] | 2.500 | 16.000 | 2.500 |
| Rmerge | 0.066 | 0.031 | 0.347 |
| Number of reflections | 25142 | 94 | 2800 |
| <I/σ(I)> | 14.18 | 37.97 | 4.06 |
| Completeness [%] | 97.8 | 94 | 97.1 |
| Redundancy | 3.53 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 11% ethylene glycol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
