4F3F
Crystal Structure of Msln7-64 MORAb-009 FAB complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2011-10-08 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.00000 |
Spacegroup name | P 64 |
Unit cell lengths | 146.163, 146.163, 80.874 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.076 - 2.650 |
R-factor | 0.1794 |
Rwork | 0.177 |
R-free | 0.22200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4f33 Difference Fourier revealed MSLN7-64 |
RMSD bond length | 0.017 |
RMSD bond angle | 1.584 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX ((phenix.refine: dev_965)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.700 |
High resolution limit [Å] | 2.610 | 2.610 |
Number of reflections | 29264 | |
<I/σ(I)> | 1.76 | |
Completeness [%] | 97.8 | 80 |
Redundancy | 2.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 294 | 0.1 M Trisodium citrate pH 5.6, 17% Polyethylene glycol 3350, VAPOR DIFFUSION, HANGING DROP, temperature 294K |