4F38

Crystal structure of geranylgeranylated RhoA in complex with RhoGDI in its active GPPNHP-bound form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	CCD
Collection date	2011-11-25
Detector	ADSC QUANTUM 210
Wavelength(s)	0.9537
Spacegroup name	P 21 21 21
Unit cell lengths	46.000, 71.576, 136.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	68.010 - 2.800
R-factor	0.20579
Rwork	0.202
R-free	0.28133
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1doa
RMSD bond length	0.015
RMSD bond angle	1.743
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASES
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	68.010	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.069	0.265
Number of reflections	20741
<I/σ(I)>	12.33	3.6
Completeness [%]	97.0	98.1
Redundancy	2.7	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	20% (w/v) PEG 3350, 0.2M MgCl2, 0.1M Hepes-NaOH, 25mM Hepes-NaOH pH 7.2, 40mM NaCl, 3mM MgCl2, 10M GMPPNP, 1mM TCEP, VAPOR DIFFUSION, HANGING DROP, temperature 293K