4EUW

Crystal structure of a HMG domain of transcription factor SOX-9 bound to DNA (SOX-9/DNA) from Homo sapiens at 2.77 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Collection date	2011-07-21
Detector	MARMOSAIC 325 mm CCD
Spacegroup name	C 2 2 2
Unit cell lengths	55.772, 134.654, 49.987
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	27.920 - 2.770
R-factor	0.24826
Rwork	0.247
R-free	0.27835
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3f27
RMSD bond length	0.009
RMSD bond angle	1.399
Data reduction software	XDS
Data scaling software	XSCALE (December 6, 2010)
Phasing software	PHASER (2.3.0)
Refinement software	REFMAC (5.7.0127)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	27.922	27.920	2.840
High resolution limit [Å]	2.770	12.390	2.770
Rmerge	0.066	0.016	0.575
Total number of observations	18522
Number of reflections	4880	61	351
<I/σ(I)>	15.3	38.4	2.6
Completeness [%]	96.6	87.8	98.8
Redundancy	3.8	3.08	3.99

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	30.00% polyethylene glycol 8000, 0.20M ammonium acetate, 0.010 M magnesium acetate, 0.1M sodium cacodylate pH 7.0, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K