4ELM
Crystal structure of the mouse CD1d-lysosulfatide-Hy19.3 TCR complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.3 |
| Synchrotron site | ALS |
| Beamline | 5.0.3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-11-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9765 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 98.514, 126.996, 104.349 |
| Unit cell angles | 90.00, 110.53, 90.00 |
Refinement procedure
| Resolution | 92.260 - 3.480 |
| R-factor | 0.21163 |
| Rwork | 0.209 |
| R-free | 0.26494 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.105 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0104) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.260 | 3.690 |
| High resolution limit [Å] | 3.480 | 3.500 |
| Rmerge | 0.240 | 0.814 |
| Number of reflections | 30877 | |
| <I/σ(I)> | 8.3 | 2.8 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 8.3 | 8.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | 11% PEG4000, 4% tacsimate, pH 6, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
