4EHP
Crystal Structure of human vinculin head domain (residues 1-252) in complex with alpha-catenin (residues 277-382)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-10-21 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 57.250, 73.960, 107.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 22.710 - 2.660 |
| R-factor | 0.1944 |
| Rwork | 0.193 |
| R-free | 0.22940 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ibf |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.060 |
| Data scaling software | SCALA (3.3.16) |
| Phasing software | MOLREP |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 107.432 | 107.432 | 2.800 |
| High resolution limit [Å] | 2.655 | 8.400 | 2.650 |
| Rmerge | 0.089 | 0.018 | 0.485 |
| Total number of observations | 3092 | 14365 | |
| Number of reflections | 13785 | ||
| <I/σ(I)> | 21.2 | 25.5 | 1.6 |
| Completeness [%] | 100.0 | 99.9 | 100 |
| Redundancy | 7.2 | 6.1 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 295 | PEG 3350, sodium acetate, VAPOR DIFFUSION, temperature 295K |
