4E45
Crystal structure of the hMHF1/hMHF2 Histone-Fold Tetramer in Complex with Fanconi Anemia Associated Helicase hFANCM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-09 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.033180 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 110.980, 69.970, 116.050 |
| Unit cell angles | 90.00, 91.60, 90.00 |
Refinement procedure
| Resolution | 49.510 - 2.000 |
| R-factor | 0.204 |
| Rwork | 0.202 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1taf |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.520 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 49.510 | 2.050 | |
| High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
| Rmerge | 0.052 | 0.033 | 0.512 |
| Number of reflections | 119106 | 872 | 8864 |
| <I/σ(I)> | 14.9 | 29.3 | 3 |
| Completeness [%] | 98.9 | 60.3 | 99.7 |
| Redundancy | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.8 | 298 | Tracking Code C4, 0.1M Tris pH 7.8, 0.2M LiCl, 0.1M Na2SO4, 17.5% w/v PEG3350, 10% glycerol cryo., vapor diffusion, sitting drop, temperature 298K |
