4E41
Structural basis for the recognition of mutant self by a tumor-specific, MHC class II-restricted T cell receptor G4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 126.133, 175.611, 88.646 |
| Unit cell angles | 90.00, 110.75, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.600 |
| R-factor | 0.20076 |
| Rwork | 0.197 |
| R-free | 0.26374 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2iam |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.687 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.700 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.269 | |
| Number of reflections | 53716 | |
| <I/σ(I)> | 2.6 | |
| Completeness [%] | 97.2 | 83.8 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 298 | 20% (wt/vol) polyethylene glycol 1000, 0.2 M calcium acetate, 0.1 M imidazole, pH 8.0, VAPOR DIFFUSION, temperature 298K |
