4E3X

Crystal Structure of Mus musculus 1-pyrroline-5-carboxylate dehydrogenase cryoprotected in proline

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2011-08-18
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97924
Spacegroup name	P 21 21 21
Unit cell lengths	84.980, 93.878, 132.406
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.479 - 1.240
R-factor	0.1562
Rwork	0.155
R-free	0.18030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3v9j
RMSD bond length	0.005
RMSD bond angle	1.078
Data reduction software	XDS
Data scaling software	SCALA (3.3.16)
Refinement software	PHENIX (1.7.3_928)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.639	45.639	1.310
High resolution limit [Å]	1.240	3.920	1.240
Rmerge		0.032	0.550
Total number of observations		44859	123860
Number of reflections	279378
<I/σ(I)>	15	15.7	1.4
Completeness [%]	94.1	97.6	87.3
Redundancy	4.1	4.6	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.25	294	20% PEG 3350, 0.1 M Bis-Tris, pH 6.25, VAPOR DIFFUSION, SITTING DROP, temperature 294K