4DX1
Crystal structure of the human TRPV4 ankyrin repeat domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-E |
| Synchrotron site | APS |
| Beamline | 24-ID-E |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-17 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 53.370, 53.370, 440.711 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.120 - 2.850 |
| R-factor | 0.2184 |
| Rwork | 0.215 |
| R-free | 0.27850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.513 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.000 | 40.000 | 2.900 |
| High resolution limit [Å] | 2.850 | 7.720 | 2.850 |
| Rmerge | 0.115 | 0.049 | 0.641 |
| Number of reflections | 18334 | ||
| <I/σ(I)> | 12.3 | ||
| Completeness [%] | 99.6 | 98.9 | 99.3 |
| Redundancy | 5.3 | 4.8 | 5.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 277 | 0.35M sodium potassium phosphate, 0.1M HEPES, 10% glycerol, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
