4DDG

Crystal structure of human OTUB1/UbcH5b~Ub/Ub

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	198.25
Detector technology	CCD
Collection date	2011-12-12
Detector	RAYONIX MX-300
Wavelength(s)	0.97949
Spacegroup name	P 1 21 1
Unit cell lengths	134.566, 104.928, 148.479
Unit cell angles	90.00, 104.19, 90.00

Refinement procedure

Resolution	49.121 - 3.299
R-factor	0.2356
Rwork	0.233
R-free	0.28020
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ESK AND 2ZFY
RMSD bond length	0.004
RMSD bond angle	0.938
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.7.1_743))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.290	3.500
High resolution limit [Å]	3.299	3.299
Number of reflections	60387
Completeness [%]	99.5	97.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	25% PEG1500, 0.1 M SPG, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K