4D9Q
Inhibiting Alternative Pathway Complement Activation by Targeting the Exosite on Factor D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-11 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 182.330, 80.760, 142.630 |
| Unit cell angles | 90.00, 107.19, 90.00 |
Refinement procedure
| Resolution | 33.380 - 2.280 |
| R-factor | 0.1911 |
| Rwork | 0.189 |
| R-free | 0.23010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1hfd |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.170 |
| Data scaling software | SCALA (3.3.16) |
| Refinement software | BUSTER-TNT (BUSTER 2.11.2) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 73.267 | 73.267 | 2.400 |
| High resolution limit [Å] | 2.280 | 7.210 | 2.280 |
| Rmerge | 0.014 | 0.399 | |
| Number of reflections | 90191 | ||
| <I/σ(I)> | 17.3 | 42.6 | 2 |
| Completeness [%] | 99.8 | 99.4 | 99.9 |
| Redundancy | 3.7 | 3.6 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 292 | 0.1 M MES pH 6.5, 25% PEG 550 MME, 0.01 M zinc sulfate and 3% 6-aminohexanoic acid, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
